Proteolytic Activity from an Alkali-Thermotolerant Streptomyces gulbargensis sp. nov.

S. G. Dastager Æ Agasar Dayanand Æ Wen-Jun Li Æ Chang-Jin Kim Æ Jae-Chan Lee Æ Dong-Jin Park Æ Xin-Peng Tian Æ Q. S. Raziuddin Received: 9 April 2007 / Accepted: 13 January 2008  Springer Science+Business Media, LLC 2008 Abstract Multiple proteases were produced and partially purified from an alkali-thermotolerant novel species of Streptomyces (i.e., Streptomyces gulbargensis DAS 131) after 48 h of growth at 45C. The enzyme preparation exhibited activity over a broad range of pH (4–12) and temperature (27–55C). Optimum activity was observed at a pH of 9.0 and a temperature of 45C. Starch and protease peptone was found to be a good source of carbon and nitrogen to enhance the enzyme...

ANTIMICROBIAL ATTRIBUTES OF RARE ACTINOBACTERIA DETECTED FROM LIMESTONE QUARRIES

SYED RAZIUDDIN QUADRI AND DAYANAND AGSAR Rare actinobacterial isolates belonging to the genera Micromonospora, Nonomuraea, Kribbella, Lechevalieria, Saccharotherix and commonly occurring Streptomyces were isolated from Limestone quarry - a unique and geographical area and harsh habitat. pH – 9.0, temperature – 45°C and sodium chloride concentration – 3% were found to be optimum for the maximum growth and physiological ability of the test isolates indicating them as thermo-alkaliphilic in nature. It was found that, the test isolate DRQ 10 belonging to the genus Streptomyces and DRQ 72 belonging to the genus Micromonospora were very effective showing maximum zone of inhibition against the...

Detection of melanin producing thermo-alkaliphilic Streptomyces from limestone quarries of the Deccan traps

Syed Raziuddin Quadri and Dayanand Agsar Streptomyces is a prominent genus under actinomycetes and well known for the synthesis of variety of bioactive molecules. In the present study, eight Streptomyces species were isolated and characterized from the soil samples of limestone quarries of the Deccan traps, India. The identified organisms being thermo-alkaliphilic, alkaline pH (9.0), higher temperature (45oC) and 3.0 % concentration of sodium chloride were proved to be important physiological attributes for the more synthesis of melanin. DRQ 11, 12 and 26 have been proved as efficient isolates for the increased synthesis of melanin - 2.76 µg/ml, 2.66 µg/ml and 2.85 µg/ml in Shinobu's...

Pontibacter niistensis sp. nov., isolated from forest soil

Syed G. Dastager, 1 Q. S. Raziuddin,2 C. K. Deepa,1 Wen-Jun Li3 and Ashok Pandey1 A Gram-negative, rod-shaped bacterial strain, NII-0905 T, that was motile by gliding was isolated from soil of a dense forest collected from the Western Ghats of India and its taxonomic position was established. Strain NII-0905T contained MK-7 as the major menaquinone and anteiso-C17 : 0, anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major cellular fatty acids. The DNA G+C content of strain NII-0905 T was 51.47 mol%. 16S rRNA gene sequence-based phylogenetic analysis confirmed the placement of strain NII-0905T in the genus Pontibacter and strain NII-0905T exhibited 93.9–96.3 % 16S rRNA sequence similarity...

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Agarwal A, Rao A M, Alhazimi A M, et al. (June 04, 2023) Integrating High-Impact Practices (HIPs) In Medical Curriculum At Northern Border University: Strengths, Challenges, and Examples. Cureus 15(6): e39938. DOI 10.7759/cureus.39938 S, A. M. R., & Agarwal, A. (2024). Recent Trends and Practices in Blood Transfusion. International Journal of Medical Science And Diagnosis Research, 8(1). https://doi.org/10.32553/ijmsdr.v8i1.1005